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|Sample type||Average %||Range|
|Cell culture supernatant||93.26||86% - 104%|
|Serum||92.79||85% - 104%|
|Plasma||94.65||84% - 103%|
Abcam’s TIMP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat TIMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat TIMP- 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-rat TIMP1||2 vials|
|Recombinant rat TIMP1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TIMP1 Microplate (12 x 8 wells)||1 unit|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100782 has not yet been referenced specifically in any publications.