• Product nameRat TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    82.3 pg/ml - 20000 pg/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 93.17 81% - 105%
    Tissue Extracts 92.48 80% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s TNF alpha ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF alpha in cell lysates and tissue lysates.

    This assay employs an antibody specific for Rat TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat TNF alpha ELISA Kit images

  • Rat TNF alpha detected in cell lysates from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma). Results shown for TNF alpha levels in 10e6 cells after background signal was subtracted (duplicates +/- SD).

  • Rat TNF alpha detected in cell supernatants from NR8383.1 control cells (C) or cells stimulated for 6 hours with 5 ug x mL-1 of LPS (Sigma); background signal subtracted (duplicates +/- SD).

  • Representative standard curve using ab100785


References for Rat TNF alpha ELISA Kit (ab100785)

This product has been referenced in:
  • Margonis GA  et al. Effectiveness of sildenafil and U-74389G in a rat model of colitis. J Surg Res 193:667-74 (2015). Read more (PubMed: 25277360) »
  • Kemse NG  et al. A combined supplementation of omega-3 fatty acids and micronutrients (folic acid, vitamin B12) reduces oxidative stress markers in a rat model of pregnancy induced hypertension. PLoS One 9:e111902 (2014). Rat . Read more (PubMed: 25405347) »

See all 3 Publications for this product

Product Wall

Yes, that lysis buffer will be good, the 150 mM sodium chloride, 1.0% Triton X-100, 50 mM Tris pH 8.0.

Here are some general guidelines.

Avoid using >0.1% SDS or other strongly denaturing detergents. In general, non-ionic de...

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Thank you for contacting us. The cell lysis buffer does not contain any protease inhibitor. Please let me know if you have any further questions.

I can suggest the following general tissue lysate preparation procedure to prepare samples for both the kits.

Tissue lyste samples can be prepared using most conventional methods, for example with RIPA or other formula...

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Thank you for your enquiry.

Please rest assured that there are no hazardous components in this kit according to Japanese law, and is safe for use by customers in Japan.

Have a nice day!

Abreview for TNF alpha Rat ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Rat Tissue lysate - whole (spinal cord)
Specification: spinal cord
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Traumatized segment level T13
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: I have used the kit with fresh rat spinal cord samples that underwent compression. The samples where homogenized a day before and kept in -20c. The strips allowed me to use the kit 3 times. The third time was a week after I have open Item F and still got a nice curve. R-square=0.9959.
Results are more than satisfying. It is a quick and straight forward assay.

Graph labels: Y axis is absorbance, X axis is pg/ml.The key labels are, from top to bottom: Standards, suppresses stds, standard curve.

Prof. Guy Sovak

Verified customer

Submitted May 21 2012

Thank you for your enquiry.

The lysis buffer (item J) is used to prepare the cell and tissue sample. There is no mention on how to prepare your sample in the Protocol Booklet as most laboratories would have their own protocols and methods fo...

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