Overview

  • Product nameRat TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 12 543.5pg/ml 44.7 8.2%
    B 10 130.1pg/ml 10.3 7.9%
    Inter-assay
    Sample n Mean SD CV%
    A 9 541.4pg/ml 33.2 6.1%
    B 9 149.4pg/ml 9.8 6.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.

    A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

    This kit will recognize both endogenous and recombinant rat TNF alpha.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in diseaseGenetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat TNF alpha ELISA Kit images

  • Rat TNF alpha measured in culture supernatants (dilution range 1/1-1/200) from control NR8383 cells or cells stimulated for 24 hours with 50 ng x mL-1 of PMA ((PMA) ab120297) or PMA with the addition of 1 ug x mL-1 of LPS (Sigma) (LPS+PMA) for the last 6 hours (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab46070

Protocols

References for Rat TNF alpha ELISA Kit (ab46070)

This product has been referenced in:
  • Kang SJ  et al. Inhibitory effects of Persicariae Rhizoma aqueous extracts on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats. Exp Ther Med 12:1563-1571 (2016). Rat . Read more (PubMed: 27588077) »
  • Jiang YH  et al. Cardiac Dysregulation and Myocardial Injury in a 6-Hydroxydopamine-Induced Rat Model of Sympathetic Denervation. PLoS One 10:e0133971 (2015). Read more (PubMed: 26230083) »

See all 6 Publications for this product

Product Wall

Thank you for your reply.

I look forward to receiving the resuls and order number for the ab100786 VEGF rat ELISA kit.

For the TNF kit ab46070, I think it is likely to be the sample that is the problem.However, I would be happy to...

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Thank you for your message. I look forward to receiving the further information regarding ab100786 VEGF Rat ELISA Kitso we can investigate this further for you.

I am sorry to hear you also have some concerns regarding two other kits. I would...

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Thank you for contacting us.

I have been in contact with the production group regarding your inquiry. Unfortunately, at this time, we do not offer individual components of this kit for sale. Information about capture antibody used to coat the...

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Vielen Dank für Ihre Anfrage.

Wie versprochen habe ich mich mit dem Hersteller und unserer Produktabteilung in Verbindung gesetzt, um zu erfahren, ob es das Streptavidin-HRP separat erhältlich gibt. Leider haben wir diese Einzelkomponente vo...

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Thank you for contacting us. The limit of detection of the TNF alpha ELISA kit ab46070 is 15 pg/ml, which is arrived at by taking the mean optical density of 64 blank wells (no TNF alpha), adding two standard deviations of the mean, then calculating...

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Abreview for TNF alpha Rat ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews
Sample: Rat Serum (rat serum)
Specification: rat serum
Amount of Sample: 0.1 µg
Type of Kit Used: ELISA
Positive control: Serial dilution of target
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Concentration estimated as serum was used.
Username

Mrs. Rhoda Cortes

Verified customer

Submitted Aug 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"