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Abcam’s TNFα Rat in vitro ELISA Set is designed for the quantitative measurement of Rat TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Rat TNF alpha Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml
|Components||Identifier||5 x 96 tests||10 x 96 tests||15 x 96 tests|
|Detection biotinylated anti-rat TNF alpha antibody||1 x 0.55ml||2 x 0.55ml||3 x 0.55ml|
|Rat TNF alpha Capture antiboy||1 x 0.5ml||2 x 0.5ml||3 x 0.5ml|
|TNF alpha Standard (Lyophilized)||Yellow||5 vials||10 vials||15 vials|
|Chromogen TMB Substrate Solution||2 vials||4 vials||6 vials|
|Streptavidin-HRP||1 vial||2 vials||3 vials|
Our Abpromise guarantee covers the use of ab48419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab48419 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"