Rat VEGF ELISA Kit (ab100786)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 0.82 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
-
Product name
Rat VEGF ELISA Kit
See all VEGFA kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
0.82 pg/ml - 200 pg/ml -
Recovery
97 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.26 84% - 103% Serum 97.43 83% - 103% Plasma 96.42 85% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat -
Product overview
Abcam’s VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat VEGF in serum, plasma, cellculture supernatants.
This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Rat VEGF 2 vials Recombinant rat VEGF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml VEGF Microplate (12 x 8 wells) 1 unit -
Research areas
-
Function
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
Tissue specificity
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
Involvement in disease
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Cellular localization
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
-
Alternative names
- Folliculostellate cell-derived growth factor
- Glioma-derived endothelial cell mitogen
- MGC70609
see all -
Database links
- Entrez Gene: 83785 Rat
- SwissProt: P16612 Rat
- Unigene: 1923 Rat
Images
-
VEGF measured in rat spleen and rat bone marrow showing quantity (pg) per mL of tested sample. Samples were diluted 9-27 fold.
-
VEGF measured in A10 cell culture supernatant and biological fluids showing quantity (pg) per mL of tested sample. RBL-1, PC12 and SV40LT-SIV cell culture supernatants were also assayed for rat VEGF and measured > 200 pg/mL.
-
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
-
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Datasheets and documents
-
SDS download
-
Datasheet download
References (20)
ab100786 has been referenced in 20 publications.
- Jun JH et al. Combination Therapy of Placenta-Derived Mesenchymal Stem Cells with WKYMVm Promotes Hepatic Function in a Rat Model with Hepatic Disease via Vascular Remodeling. Cells 11:N/A (2022). PubMed: 35053347
- Zhou W et al. Constructing Tissue-Engineered Dressing Membranes with Adipose-Derived Stem Cells and Acellular Dermal Matrix for Diabetic Wound Healing: A Comparative Study of Hypoxia- or Normoxia-Culture Modes. Stem Cells Int 2022:2976185 (2022). PubMed: 35571531
- Cui Z et al. Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation. Bone Res 10:58 (2022). PubMed: 36031625
- Wu P et al. Co-culture with Endothelial Progenitor Cells promotes the Osteogenesis of Bone Mesenchymal Stem Cells via the VEGF-YAP axis in high-glucose environments. Int J Med Sci 18:1628-1638 (2021). PubMed: 33746579
- Sun L et al. Regulatory role of miRNA-23a in diabetic retinopathy. Exp Ther Med 22:1477 (2021). PubMed: 34765018