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|Sample type||Average %||Range|
|Cell culture extracts||92.67||81% - 102%|
|Tissue Extracts||94.34||82% - 102%|
Abcam’s VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat VEGF in cell lysates and tissue lysates.
This assay employs an antibody specific for Rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|120X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|5X Assay Diluent B||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|Biotinylated anti-rat VEGF||2 vials|
|Recombinant rat VEGF Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
|VEGF Microplate (12 x 8 wells)||1 unit|
Our Abpromise guarantee covers the use of ab100787 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|