Overview

  • Product nameRat VEGF ELISA Kit
    See all VEGFA kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    0.82 pg/ml - 200 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 92.67 81% - 102%
    Tissue Extracts 94.34 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat VEGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for Rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • Tissue specificityIsoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • Involvement in diseaseDefects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Cellular localizationSecreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • Alternative names
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VEGFA_HUMAN
    • VPF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Rat VEGF ELISA Kit images

  • Representative standard curve using ab100787

Protocols

References for Rat VEGF ELISA Kit (ab100787)

This product has been referenced in:
  • Lee EK  et al. Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model. PLoS One 9:e110109 (2014). Enzyme Immunoassay ; Rat . Read more (PubMed: 25314292) »

See 1 Publication for this product

Product Wall



The cell or tissue lysates for use can be prepared using most conventional methods, e.g. homogenization of cell or tissue in Lysis Buffer. Other general low-salt lysis buffers can be used with the following caveats:



1) Avoi...

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The BCA total protein assay will be fine with our lysis buffer. In fact, we use it all the time in our lab to determine the total protein in lysate samples. To my knowledge, the Bradford assay is the only total protein assay which is http://en.wikipedi...

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The concentration of the HRP-streptavidin reagent is considered proprietary information by the developer of the kit. I am sorry we could not be of more help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"