Overview

  • Product name
    Rat VEGF ELISA Kit
    See all VEGFA kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    0.82 pg/ml - 200 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 92.67 81% - 102%
    Tissue Extracts 94.34 82% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s VEGF Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat VEGF in cell lysates and tissue lysates.

    This assay employs an antibody specific for Rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100787

Protocols

References

This product has been referenced in:
  • Lee EK  et al. Melissa officinalis extract inhibits laser-induced choroidal neovascularization in a rat model. PLoS One 9:e110109 (2014). Rat . Read more (PubMed: 25314292) »

See 1 Publication for this product

Customer reviews and Q&As



The cell or tissue lysates for use can be prepared using most conventional methods, e.g. homogenization of cell or tissue in Lysis Buffer. Other general low-salt lysis buffers can be used with the following caveats:



1) Avoi...

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The BCA total protein assay will be fine with our lysis buffer. In fact, we use it all the time in our lab to determine the total protein in lysate samples. To my knowledge, the Bradford assay is the only total protein assay which is http://en.wikipedi...

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Thank you for contacting us.

The concentration of the HRP-streptavidin reagent is considered proprietary information by the developer of the kit. I am sorry we could not be of more help.

Please do not hesitate to contact us if you...

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I am happy to hear that the new kits are working well for you! Please let me know if there is anything else I can do to help.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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