Overview

  • Product name
  • Description
    Rabbit polyclonal to RAVER1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Chinese hamster
  • Immunogen

    Recombinant fragment, corresponding to a region between amino acids 556-606 of Human RAVER1 (UniProt ID: Q8IY67.1).

  • Positive control
    • 293T, HeLa and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 64 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Cooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
  • Sequence similarities
    Contains 3 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus. Cytoplasm. Nuclear, in perinucleolar structures. Shuttles between nucleus and cytoplasm. Cytoplasm, at focal contacts and cell-cell contacts. Associated with myotubes during muscle differentiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1978 antibody
    • Protein raver-1 antibody
    • RAVER1 antibody
    • RAVR1_HUMAN antibody
    • Ribonucleoprotein PTB-binding 1 antibody
    • ribonucleoprotein, PTB binding 1 antibody
    see all

Images

  • All lanes : Anti-RAVER1 antibody (ab157131) at 1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation with ab157131 at 6µg/mg lysate. 1 mg of 293T whole cell lysate used for IP (20% of IP loaded). Western blot labelling RAVER1 with ab157131 at 1 µg/ml. Detected by chemiluminescence with exposure time of 30 seconds.

References

ab157131 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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