Overview

  • Product nameAnti-RAVER1 antibody
    See all RAVER1 primary antibodies
  • Description
    Rabbit polyclonal to RAVER1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Cat
  • Immunogen

    A region within synthetic peptide: MPLRPGSLVP EGAGFPKMAA DVSVTHRPPL SPKSGAEVEA GDAAERRAPE, corresponding to N terminal amino acids 1-50 of Human RAVER1

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human kidney tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionCooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
  • Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
  • Cellular localizationNucleus. Cytoplasm. Nuclear, in perinucleolar structures. Shuttles between nucleus and cytoplasm. Cytoplasm, at focal contacts and cell-cell contacts. Associated with myotubes during muscle differentiation.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1978 antibody
    • Protein raver-1 antibody
    • RAVER1 antibody
    • RAVR1_HUMAN antibody
    • Ribonucleoprotein PTB-binding 1 antibody
    • ribonucleoprotein, PTB binding 1 antibody
    see all

Anti-RAVER1 antibody images

  • All lanes : Anti-RAVER1 antibody (ab69542) at 1.25 µg/ml

    Lane 1 : MW markers
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling RAVER1 with ab69542 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

References for Anti-RAVER1 antibody (ab69542)

ab69542 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"