|
|
|
|
|
Product Name
|
RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
|
|
Product type
|
Lysates
|
|
Description
|
RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
|
|
Tested applications
(see key)
|
WB
|
|
Application notes
(see key)
|
Recommended dilutions
RAW 264.7 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
|
|
Research areas
|
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Other
|
|
Relevance
|
Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.
Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
|
|
Purity
|
Whole Cell Lysate
|
|
Storage buffer
|
Preservative: None
Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 0.2% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 1mM EDTA, 1mM PMSF
|
|
Form
|
Liquid
|
|
Concentration
|
2.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor)
Growth media: DMEM & 10% fetal bovine serum
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to RAW 264.7
|
| |
RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate - more information
|
Customer reviews (feedback) regarding RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate |
Customer FAQs regarding RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate |
Protocols for RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate |
Price and availability of products related to RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate |
| |
RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate - other tools:
|
Contact Abcam with a Technical Enquiry about RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
