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Products:Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Other
Overview
Product name
RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
General notes
Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor)
Growth media: DMEM & 10% fetal bovine serum
Tested applications
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 5% Beta mercaptoethanol
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Lysate notes
Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Research areas
Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Other
Background
Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (Ready to load.)
References for RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate (ab7187)
ab7187 has not yet been referenced specifically in any publications.
Publishing research using ab7187? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"