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Product Name
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RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
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Product type
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Lysates
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Description
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RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
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Tested applications
(see key)
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WB
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Application notes
(see key)
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Recommended dilutions RAW 264.7 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Whole Cell Lysates >> Mouse >> Other
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Relevance
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Established from the ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells have receptors for immunoglobulin and produce lysozyme. They are used in metabolic, inflammation and apoptosis studies.
Mouse RAW 264.7 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
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Purity
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Whole Cell Lysate
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Storage buffer
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Preservative: None Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 0.2% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 1mM EDTA, 1mM PMSF
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Cell line: RAW 264.7 (Abelson murine leukemia virus-induced tumor) Growth media: DMEM & 10% fetal bovine serum
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