Overview

  • Product nameAnti-RAX antibody
    See all RAX primary antibodies
  • Description
    Rabbit polyclonal to RAX
  • Specificityab23340 reacts with RAX (retinal homeobox protein Rx).
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human RAX aa 1-50.
    Sequence:

    MHLPGCAPAMADGSFSLAGHLLRSPGGSTSRLHSIEAILGFTKDDGILGT


    Database link: Q9Y2V3

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab23340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPlays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation. Binds to the photoreceptor conserved element-I (PCE-1/Ret 1) in the photoreceptor cell-specific arrestin promoter.
  • Tissue specificityExpressed in the developing eye and weakly expressed in the adult retina.
  • Involvement in diseaseDefects in RAX are the cause of microphthalmia isolated type 3 (MCOP3) [MIM:611038]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present.
  • Sequence similaritiesBelongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MCOP3 antibody
    • RAX antibody
    • retina and anterior neural fold homeobox antibody
    • Retina and anterior neural fold homeobox protein antibody
    • Retinal homeobox protein Rx antibody
    • RX antibody
    • RX_HUMAN antibody
    see all

Anti-RAX antibody images

  • Anti-RAX antibody (ab23340) at 1 µg/ml + Jurkat cell lysate

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ab23340 at 4.0 µg/ml staining human spermatophore by IHC-P.  Epithelial cells, with positive label, are indicated with arrows. Magnification 400X.

References for Anti-RAX antibody (ab23340)

ab23340 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and for your patience. It has taken some time to discuss this with our source for this antibody.

It is unfortunately difficult to say what the lighter brown staining is exactly in the IHC image provided for ab23340...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"