Overview

  • Product nameAnti-Rb antibody [E182]
    See all Rb primary antibodies
  • Description
    Rabbit monoclonal [E182] to Rb
  • SpecificityThe antibody detects pan Rb protein.
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt or IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Ser780 of human Rb

  • Positive control
    • Jurkat cell lysate A431 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
IP 1/40.
WB 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 106 kDa).
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    • FunctionKey regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
    • Tissue specificityExpressed in the retina.
    • Involvement in diseaseChildhood cancer retinoblastoma
      Bladder cancer
      Osteogenic sarcoma
    • Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
    • DomainThe Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
    • Post-translational
      modifications
      Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
      N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
      Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • OSRC antibody
      • Osteosarcoma antibody
      • p105-Rb antibody
      • P105RB antibody
      • PP105 antibody
      • pp110 antibody
      • PPP1R130 antibody
      • pRb antibody
      • Prepro retinoblastoma associated protein antibody
      • Protein phosphatase 1 regulatory subunit 130 antibody
      • Rb antibody
      • RB_HUMAN antibody
      • RB1 antibody
      • RB1 gene antibody
      • Retinoblastoma 1 antibody
      • Retinoblastoma suspectibility protein antibody
      • Retinoblastoma-associated protein antibody
      see all

    Anti-Rb antibody [E182] images

    • All lanes : Anti-Rb antibody [E182] (ab32513) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cell lysate
      Lane 2 : Jurkat cell lysate treated with FBS
      Lane 3 : Jurkat cell lysate treated with Alkaline Phosphatase.


      Predicted band size : 106 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of (A) non-treated and (B) Lambda Phosphatase-treated A431 cells using anti-Phospho-Rb (S780) ab32513 Rabbit Monoclonal Antibody

    References for Anti-Rb antibody [E182] (ab32513)

    This product has been referenced in:
    • Richardson E  et al. Mechanism-based screen establishes signalling framework for DNA damage-associated G1 checkpoint response. PLoS One 7:e31627 (2012). ICC/IF ; Human . Read more (PubMed: 22384045) »
    • Haller F  et al. Increased KIT signalling with up-regulation of cyclin D correlates to accelerated proliferation and shorter disease-free survival in gastrointestinal stromal tumours (GISTs) with KIT exon 11 deletions. J Pathol 216:225-35 (2008). Read more (PubMed: 18729075) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    I have received the answers to your questions:

    1) Lambda phosphatase is usedafter fixation.
    2) Unfortunately, we do not have any product references at this time.

    3) The Lambda phosphatase...

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    Thank you for calling Abcam earlier today.
    I am sorry about the issues you were having with ab47763 in western blotting. As we discussed, I am sending you an aliquot of ab32513 as a free of charge replacement. Your new order number is *.
    If the...

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    Thank you for contacting us.

    I have had the opportunity to discuss this with the lab. Unfortunately, we have not tested whether this product would recognize the hyperphosphoylated Rb protein. We cannot guarantee that it will work.
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    Thank you for contacting us.

    I have heard back from the lab with the following information:

    We found negative results on a cancer TMA, but no other details are available.
    The concentration varies from lot to lot, but the same ...

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    Thank you for your patience as I have obtained the following information from the laboratory:


    Regarding the antibody being unsuitable for IHC, we found negative staining using a cancer TMA. No additional troubleshooting was done. I woul...

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    Thank your calling Abcam earlier today. The concentration of ab32513, lot #GR12953-2 is ******* mg/mL. Please letme know if there is anything else I can help you with.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"