Overview

  • Product nameAnti-Rb antibody [Rb1 1F8]
    See all Rb primary antibodies
  • Description
    Mouse monoclonal [Rb1 1F8] to Rb
  • SpecificityThis antibody reacts with hyperphosphorylated and un (under) phosphorylated forms of Rb protein.
  • Tested applicationsSuitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Chimpanzee
  • Immunogen

    Rb-b-galactosidase fusion protein spanning nucleotides 1126-1973 of human Rb gel purified from bacterial lysates (R. Grand et al. 1989).

  • EpitopeThe epitope has been mapped between aa 703-772 of human RB1.
  • Positive control
    • Tested in a panel of human cell lines and CV-1 cell line (established from monkey kidney epithelium).

Properties

Applications

Our Abpromise guarantee covers the use of ab24 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionKey regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
  • Tissue specificityExpressed in the retina.
  • Involvement in diseaseChildhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma
  • Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
  • DomainThe Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.
  • Post-translational
    modifications
    Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OSRC antibody
    • Osteosarcoma antibody
    • p105-Rb antibody
    • P105RB antibody
    • PP105 antibody
    • pp110 antibody
    • PPP1R130 antibody
    • pRb antibody
    • Prepro retinoblastoma associated protein antibody
    • Protein phosphatase 1 regulatory subunit 130 antibody
    • Rb antibody
    • RB_HUMAN antibody
    • RB1 antibody
    • RB1 gene antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma suspectibility protein antibody
    • Retinoblastoma-associated protein antibody
    see all

Anti-Rb antibody [Rb1 1F8] images

  • Anti-Rb antibody [Rb1 1F8] (ab24) at 1/1000 dilution + Human Fibroblast cell lysate(nuclear). 16 hour incubation.

    Secondary
    NIF 825 (provided in ECL kit) - HRP conjugated
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 105 kDa
    Observed band size : 100-150 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    See Abreview



  • Predicted band size : 105 kDa

    Image from Lee DY et al, PLoS One 5:e13599 (2010), Fig 3.

References for Anti-Rb antibody [Rb1 1F8] (ab24)

This product has been referenced in:
  • Araki K  et al. Cytoplasmic translocation of the retinoblastoma protein disrupts sarcomeric organization. Elife 2:e01228 (2013). IHC-Fr ; Human . Read more (PubMed: 24302570) »
  • Lee KS  et al. Helicobacter pylori CagA triggers expression of the bactericidal lectin REG3? via gastric STAT3 activation. PLoS One 7:e30786 (2012). WB . Read more (PubMed: 22312430) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lung and foreskin fibroblast cells; MOUSE lung)
Loading amount 30 µg
Specification lung and foreskin fibroblast cells; MOUSE lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 10 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Specification Hela
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mrs. Bernadette Ferraro

Verified customer

Submitted Dec 19 2005

Thank you for your enquiry. Ab24 is predicted to react with rat Rb (97% identity with immunogen) due to sequence homology but this has not been specifically tested yet. Should you decide to go ahead and purchase this product, please let us know how ...

Read More

Thank you for your enquiry. Ab24 also recognises the phosphorylates and unphospohorylated forms of Rb1, so it is nonspecific. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (human fibroblast)
Specification human fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Oct 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"