Anti-RB associated KRAB repressor antibody (ab28487)

Overview

  • Product name
    Anti-RB associated KRAB repressor antibody
    See all RB associated KRAB repressor primary antibodies
  • Description
    Rabbit polyclonal to RB associated KRAB repressor
  • Specificity
    ab28487 recognises RB associated KRAB repressor ((RBAK))
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from residues 447-496 of human RB associated KRAB repressor.

    .

  • Positive control
    • Raji cell lysate, human kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab28487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2500.
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May repress E2F-dependent transcription. May promote AR-dependent transcription.
  • Tissue specificity
    Expressed in bone, brain, heart, kidney, liver, lung, pancreas and placenta.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 16 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hRBaK antibody
    • Hypothetical protein RBAK antibody
    • RB associated KRAB zinc finger antibody
    • RB-associated KRAB repressor antibody
    • RB-associated KRAB zinc finger protein antibody
    • RBAK antibody
    • RBAK_HUMAN antibody
    • Zinc finger protein 769 antibody
    • ZNF 769 antibody
    • ZNF769 antibody
    see all

Images

  • Anti-RB associated KRAB repressor antibody (ab28487) at 2 µg/ml (in 5% skim milk / PBS buffer) + Raji cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of RB associated KRAB repressor in paraffin embedded human kidney tissue slide using 4 µg/ml of ab28487. Arrows indicate positively labelled epithelial cells of the renal tubule.

References

ab28487 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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