Overview

  • Product name
  • Description
    Rabbit polyclonal to Rb2 p130
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    KYPQEEQPRQQRGRK

    , corresponding to amino acids 175-189 of Human Rb2 p130

  • Positive control
    • HeLa and Jurkat T cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

Target

  • Relevance
    Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • 130 kDa retinoblastoma associated protein antibody
    • FLJ26459 antibody
    • p130 antibody
    • PRB 2 antibody
    • PRB2 antibody
    • Rb 2 antibody
    • Rb2 antibody
    • RBL 2 antibody
    • RBL2 antibody
    • RBR 2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma like protein 2 antibody
    • Retinoblastoma related gene antibody
    see all

Images

  • All lanes : Anti-Rb2 p130 antibody (ab71143) at 1/2000 dilution

    Lane 1 : Jurkat T cell lysate
    Lane 2 : HeLa cell lysate


    Predicted band size : 128 kDa
    Observed band size : 128 kDa

References

ab71143 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19 cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Specification
ARPE-19 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Vero cell)
Permeabilization
Yes - 0.25% Tx-100
Specification
Vero cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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