Recombinant
RabMAb

Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

Overview

  • Product name
    Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]
    See all Rb2 p130 primary antibodies
  • Description
    Rabbit monoclonal [EP2387AY] to Rb2 p130 (phospho S952)
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding serine 952 of human p130

  • Positive control
    • Jurkat cell lysate; human breast carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab68136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
  • Application notes
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250.
    WB: 1/10000 - 1/500000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

    Is unsuitable for Flow Cyt or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Two retinoblastoma related proteins, p107 and pRb2 / p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb / p105), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. These Rb family proteins were measured in a variety of lung neoplasms. The highest percentage of undetectable levels and the tightest inverse correlation with the histological grading and with PCNA expression in the most aggressive tumor types were found for pRb2 / p130, which may suggest an important role for this protein in the pathogenesis and progression of lung cancer.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • 130 kDa retinoblastoma associated protein antibody
      • FLJ26459 antibody
      • p130 antibody
      • PRB 2 antibody
      • PRB2 antibody
      • Rb 2 antibody
      • Rb2 antibody
      • RBL 2 antibody
      • RBL2 antibody
      • RBR 2 antibody
      • RBR2 antibody
      • Retinoblastoma like 2 antibody
      • Retinoblastoma like protein 2 antibody
      • Retinoblastoma related gene antibody
      see all

    Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] images

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68136 at a dilution of 1/100.
    • All lanes : Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/500000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-mouse at 1/2000 dilution

      Predicted band size : 128 kDa
      Observed band size : 128 kDa

    References for Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136)

    This product has been referenced in:
    • Divakar SK  et al. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas. Leukemia 30:86-93 (2016). Read more (PubMed: 26174628) »
    • Orlando S  et al. p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes. Nucleic Acids Res 43:6860-73 (2015). Read more (PubMed: 26071952) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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