Our Abpromise guarantee covers the use of ab3535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 22223663|
|ICC||Use a concentration of 3.33 µg/ml.|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47.6 kDa).Can be blocked with RbAp46 peptide (ab5013).|
|IHC-P||Use a concentration of 1 µg/ml.|
ab3535 staining RbAp46 in Hela cells by Immunocytochemistry/ immunofluorescence.
Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.