• Product nameAnti-RbAp46 antibody
    See all RbAp46 primary antibodies
  • Description
    Rabbit polyclonal to RbAp46
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Ferret, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residues 1 and 50 of RbAp46, (AAC50231.1).

  • Positive control
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.



Our Abpromise guarantee covers the use of ab72457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 48 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionCore histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 7 WD repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • G1/S transition control protein binding protein RbAp46 antibody
    • Histone acetyltransferase type B subunit 2 antibody
    • Histone binding protein RBBP7 antibody
    • Histone-binding protein RBBP7 antibody
    • MGC138867 antibody
    • MGC138868 antibody
    • Nucleosome remodeling factor subunit RBAP46 antibody
    • Nucleosome-remodeling factor subunit RBAP46 antibody
    • RBAP46 antibody
    • RBBP 7 antibody
    • RBBP-7 antibody
    • RBBP7 antibody
    • RBBP7_HUMAN antibody
    • Retinoblastoma binding protein 7 antibody
    • Retinoblastoma binding protein p46 antibody
    • Retinoblastoma-binding protein 7 antibody
    • Retinoblastoma-binding protein p46 antibody
    • Retinoblastoma-binding protein RbAp46 antibody
    see all

Anti-RbAp46 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling RbAp46 with ab72457 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-RbAp46 antibody (ab72457) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size : 48 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), with ab72457 used for IP at 3 µg/mg lysate, and for subsequent WB at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-RbAp46 antibody (ab72457)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for contacting us. For this antibody, ab72457, the concentration of the Tris is 50 mM. As I mentioned in my previous e-mail, the concentration can vary from one antibody to another but will typically be within the range of 10 - 50 mM. ...

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