• Product nameAnti-RbAp46 antibody [EPR5082]
    See all RbAp46 primary antibodies
  • Description
    Rabbit monoclonal [EPR5082] to RbAp46
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from residues within Human RbAp46

  • Positive control
    • HeLa, 293T, LnCaP and HepG2 cell lysates. Human prostatic hyperplasia tissue, Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionCore histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
    • Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
      Contains 7 WD repeats.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • G1/S transition control protein binding protein RbAp46 antibody
      • Histone acetyltransferase type B subunit 2 antibody
      • Histone binding protein RBBP7 antibody
      • Histone-binding protein RBBP7 antibody
      • MGC138867 antibody
      • MGC138868 antibody
      • Nucleosome remodeling factor subunit RBAP46 antibody
      • Nucleosome-remodeling factor subunit RBAP46 antibody
      • RBAP46 antibody
      • RBBP 7 antibody
      • RBBP-7 antibody
      • RBBP7 antibody
      • RBBP7_HUMAN antibody
      • Retinoblastoma binding protein 7 antibody
      • Retinoblastoma binding protein p46 antibody
      • Retinoblastoma-binding protein 7 antibody
      • Retinoblastoma-binding protein p46 antibody
      • Retinoblastoma-binding protein RbAp46 antibody
      see all

    Anti-RbAp46 antibody [EPR5082] images

    • All lanes : Anti-RbAp46 antibody [EPR5082] (ab109285) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : LnCaP cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 50 kDa
    • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue using ab109285 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ab109285 at 1/250 dilution.

    References for Anti-RbAp46 antibody [EPR5082] (ab109285)

    This product has been referenced in:
    • Ebata A  et al. Oestrogen-induced genes in ductal carcinoma in situ: their comparison with invasive ductal carcinoma. Endocr Relat Cancer 19:485-96 (2012). IHC-P ; Human . Read more (PubMed: 22569827) »

    See 1 Publication for this product