Overview

  • Product nameAnti-RbAp48 antibody [11G10]
    See all RbAp48 primary antibodies
  • Description
    Mouse monoclonal [11G10] to RbAp48
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein containing the complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

  • Positive control
    • Molt 4, HeLa cells.
  • General notes


    This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation.

Properties

Applications

Our Abpromise guarantee covers the use of ab488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47.5 kDa.Can be blocked with RbAp48 peptide (ab5014).

Dilute in 5% Milk/PBST and incubate at 4ºC overnight. Use under reducing conditions.

IP Use a concentration of 1 - 5 µg/ml.
ICC/IF 1/500 - 1/1000.

Permeabilization step recommended. Use in PBST containing 2% BSA and 10% normal serum at 4ºC overnight.

Target

  • FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF I p48 antibody
    • CAF-1 subunit C antibody
    • CAF-I 48 kDa subunit antibody
    • CAF-I p48 antibody
    • Chromatin assembly factor 1 subunit C antibody
    • Chromatin assembly factor I p48 subunit antibody
    • Chromatin assembly factor/CAF 1 p48 subunit antibody
    • Histone-binding protein RBBP4 antibody
    • MSI1 protein homolog antibody
    • Nucleosome-remodeling factor subunit RBAP48 antibody
    • NURF55 antibody
    • RbAp 48 antibody
    • RBAP48 antibody
    • RBBP-4 antibody
    • RBBP4 antibody
    • RBBP4_HUMAN antibody
    • Retinoblastoma binding protein 4 antibody
    • Retinoblastoma binding protein p48 antibody
    • Retinoblastoma-binding protein 4 antibody
    • Retinoblastoma-binding protein p48 antibody
    see all

Anti-RbAp48 antibody [11G10] images

  • Anti-RbAp48 antibody [11G10] (ab488) at 2 µg/ml + Whole cell lysates prepared from human colon adenocarcinoma SW480 cell line at 30 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47.5 kDa


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview



  • Predicted band size : 47.5 kDa

    Image from S Maenner et al, PLoS Biol 8:e1000276 (2010), Fig 9.

  • Overlay histogram showing HeLa cells stained with ab488 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab488, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-RbAp48 antibody [11G10] (ab488)

This product has been referenced in:
  • Kim JY  et al. Specificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor. Mol Cell 56:738-48 (2014). Read more (PubMed: 25453762) »
  • Minnebo N  et al. NIPP1 maintains EZH2 phosphorylation and promoter occupancy at proliferation-related target genes. Nucleic Acids Res 41:842-54 (2013). IP . Read more (PubMed: 23241245) »

See all 8 Publications for this product

Product Wall

Thank you very much for your interest in ab488. I am not sure whether you are interested in receiving more information about our testing program or if you are ready to receive the discount code, so I am sending all of the information about the program ...

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Thank you for your inquiry.

We have heard similar comments from customers.

What species are your cell lysates from? And have you pre-cleared your lysates?This would bepre-incubating the prepared lysate with the beads before commenci...

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We have previously been in contact regarding ab488, which was not providing satisfactory results.

We provided some troubleshooting tips for the protocol, and I am writing to inquire if these suggestions have helped to improve the results? I ...

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Thank you for contacting us. There are 4 isoforms of RBAP48 reported in swissprot (http://www.uniprot.org/uniprot/Q09028). The extra band may be isoform of the RBAP48. I would suggest searching literature. Further tests may be needed. The RBAP4...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW480, colorectal cancer)
Loading amount 30 µg
Specification SW480, colorectal cancer
Gel Running Conditions Reduced Denaturing (12%/4%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Embryonal carcinoma cells)
Loading amount 50 µg
Specification Embryonal carcinoma cells
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Patompon Wongtrakoongate

Verified customer

Submitted May 06 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (myometrium)
Specification myometrium
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted Jul 13 2006

Thank you for your patience. The originator of ab3535 has said that the antibody should not cross react with the RbAP48 protein. Please contact us again if you have any additional questions.

Thank you for your enquiry. For ab86 and ab488, the antibodies have not been tested against RbAp46 so we do not know whether or not the antibodies would react with it. I'm still waiting for information on ab3535 and will let you know when I receive.

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Thank you for your email and for updating us with your results so far. Once you have more information, please do follow-up with me. I look forward to hearing from you again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"