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Product name
Anti-RbAp48 antibody
See all RbAp48 products (13) ...
Description
Rabbit polyclonal to RbAp48
Tested applications
ICC/IF, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chicken, Xenopus laevis
Immunogen
Synthetic peptide: CENIYNDEDPEGSVDPEGQGS, corresponding to amino acids 406-425 of Human and Mouse CAF 1 (p48).
CENIYNDEDPEGSVDPEGQGS
General notes
Locus link: 350005
Alternative names: similar to chromatin assembly factor 1 p48 subunit
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.02% sodium azide
Purity
Whole antiserum
Primary antibody notes
A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Rb
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> ISWI/NURF/CHRAC
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: use at a dilution of 1/50.
IP: Use at a concentration of 20 µg/ml.
WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 47.6 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Sequence similarities
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 6 WD repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ09028
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAF 1 subunit C antibody
- CAF I 48 kDa subunit antibody
- CAF I p48 antibody
see all
Database links
- Entrez Gene: 5928 Human
- Entrez Gene: 19646 Mouse
- Omim: 602923 Human
- SwissProt: Q09028 Human
- SwissProt: Q60972 Mouse
- Unigene: 16003 Human
- Unigene: 12145 Mouse
Anti-RbAp48 antibody images:
Western blot - p48 CAF1 antibody (ab1765)
Anti-RbAp48 antibody (ab1765) at 1/1000 dilution
Secondary
Anti-rabbit IgG HRP
developed using the ECL technique
Predicted band size : 47.6 kDa
Observed band size : 40,55 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
ab1765 detecting p48 CAF1 from human LCL whole cell lysates by Western Blot.
Immunoprecipitation - p48 CAF1 antibody (ab1765)
ab1765 used in Immunoprecipitation of HeLa whole cell lysate.
Whole cell extract (containing 100µg of soluble protein) was incubated with 20µg of antibody overnight. Protein A-Agarose was added to the immunocomplexes and incubated at room temperature for three hours. Immunocomplexes were eluted and then resolved using 7.5% SDS-PAGE under reducing conditions. For the detection of HDAC1, 2 and 3 a non-Abcam mouse monoclonal antibody was used at a dilution of 1/800. Results support the understanding that HDAC1 and 2 are in the same complex together with p48. Note that HDAC1 does not coprecipitate HDAC2, probably displaced by the antibody during the experiment.
Lane 1: Input
Lane 2: HDAC1 (ab1769)
Lane 3: p48 (ab1765)
Lane 4: No antibody control
This image is courtesy of an Abreview by Hugh Spotswood submitted on 9 February 2006.
Immunocytochemistry/ Immunofluorescence - p48 CAF1 antibody (ab1765)
ab1765 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
References for Anti-RbAp48 antibody (ab1765)
This product has been referenced in:
- Pegoraro G et al. Ageing-related chromatin defects through loss of the NURD complex. Nat Cell Biol 11:1261-7 (2009). ICC/IF; Human.Read more (PubMed: 19734887) »
- Nitzsche A et al. Temporal Dynamics of Cytomegalovirus Chromatin Assembly in Productively Infected Human Cells. J Virol : (2008). WB, ICC/IF; Human.Read more (PubMed: 18786996) »
See all 2 publications for this product
Publishing research using ab1765? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"