Overview

  • Product nameAnti-RbAp48 antibody
    See all RbAp48 primary antibodies
  • Description
    Rabbit polyclonal to RbAp48
  • Tested applicationsSuitable for: ICC/IF, WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RbAp48.

    (Peptide available as ab38404.)

  • Positive control
    • ab38135 gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line)

Properties

Applications

Our Abpromise guarantee covers the use of ab38135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 48 kDa).
ChIP Use at an assay dependent concentration. PubMed: 25051498

Target

  • FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 6 WD repeats.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAF I p48 antibody
    • CAF-1 subunit C antibody
    • CAF-I 48 kDa subunit antibody
    • CAF-I p48 antibody
    • Chromatin assembly factor 1 subunit C antibody
    • Chromatin assembly factor I p48 subunit antibody
    • Chromatin assembly factor/CAF 1 p48 subunit antibody
    • Histone-binding protein RBBP4 antibody
    • MSI1 protein homolog antibody
    • Nucleosome-remodeling factor subunit RBAP48 antibody
    • NURF55 antibody
    • RbAp 48 antibody
    • RBAP48 antibody
    • RBBP-4 antibody
    • RBBP4 antibody
    • RBBP4_HUMAN antibody
    • Retinoblastoma binding protein 4 antibody
    • Retinoblastoma binding protein p48 antibody
    • Retinoblastoma-binding protein 4 antibody
    • Retinoblastoma-binding protein p48 antibody
    see all

Anti-RbAp48 antibody images

  • All lanes : Anti-RbAp48 antibody (ab38135) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat whole cell lysate (ab7899)
    Lane 4 : A431 whole cell lysate (ab7909)
    Lane 5 : HEK293 whole cell lysate (ab7902)
    Lane 6 : HepG2 whole cell lysate (ab7900)
    Lane 7 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab38135 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38135, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-RbAp48 antibody (ab38135)

This product has been referenced in:
  • Fujita T & Fujii H Identification of proteins associated with an IFN?-responsive promoter by a retroviral expression system for enChIP using CRISPR. PLoS One 9:e103084 (2014). ChIP ; Human . Read more (PubMed: 25051498) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Expressed & purified from infected insect cells)
Specification Expressed & purified from infected insect cells
Gel Running Conditions Reduced Denaturing (4-12% NuPAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Wei Zhang

Verified customer

Submitted Apr 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"