1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family. Contains 6 WD repeats.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ewing sarcoma tissue labelling RbAp48 with ab74188 at 1/200 (1µg/ml). Detection: DAB.
Western blot - RbAp48 antibody (ab74188)
All lanes : Anti-RbAp48 antibody (ab74188) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of RbAp48 by Western Blot of Immunprecipitate.
ab74188, at 0.1µg/ml, staining RbAp48 in HeLa whole cell lysate immunoprecipitated using ab74188 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.