Overview

  • Product nameAnti-RbBP5 antibody
    See all RbBP5 primary antibodies
  • Description
    Rabbit polyclonal to RbBP5
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide mapping to a region between a.a 500 and C-terminus (538) of human RbBP5.

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab52084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.

Target

  • FunctionAs part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesContains 6 WD repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • RBBP 5 antibody
    • RBBP-5 antibody
    • RBBP5 antibody
    • RBBP5_HUMAN antibody
    • RBQ 3 antibody
    • RBQ3 antibody
    • Retinoblastoma binding protein 5 antibody
    • Retinoblastoma binding protein RBQ3 antibody
    • Retinoblastoma binding protein5 antibody
    • Retinoblastoma-binding protein 5 antibody
    • Retinoblastoma-binding protein RBQ-3 antibody
    • SWD1 antibody
    • SWD1, Set1c WD40 repeat protein, homolog antibody
    see all

Anti-RbBP5 antibody images

  • ICC/IF image of ab52084 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RbBP5 antibody (ab52084)

This product has been referenced in:
  • Benedikt A  et al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB ; Human . Read more (PubMed: 21030982) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 40 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted Dec 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"