Overview

  • Product name
  • Description
    Rabbit polyclonal to RbBP5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human RbBP5.

    (Peptide available as ab87828.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab80074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).

Target

  • Function
    As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 6 WD repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • RBBP 5 antibody
    • RBBP-5 antibody
    • RBBP5 antibody
    • RBBP5_HUMAN antibody
    • RBQ 3 antibody
    • RBQ3 antibody
    • Retinoblastoma binding protein 5 antibody
    • Retinoblastoma binding protein RBQ3 antibody
    • Retinoblastoma binding protein5 antibody
    • Retinoblastoma-binding protein 5 antibody
    • Retinoblastoma-binding protein RBQ-3 antibody
    • SWD1 antibody
    • SWD1, Set1c WD40 repeat protein, homolog antibody
    see all

Images

  • All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml

    Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 60,65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References

ab80074 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80074.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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