• Product nameAnti-RbBP5 antibody
    See all RbBP5 primary antibodies
  • Description
    Rabbit polyclonal to RbBP5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 432-481 (ASSEKKRQSS ADGSQPPKKK PKTTNIELQG VPNDEVHPLL GVKGDGKSKK) of Human RbBP5, NP_005048

  • Positive control
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab87190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40, 65 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionAs part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesContains 6 WD repeats.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • RBBP 5 antibody
    • RBBP-5 antibody
    • RBBP5 antibody
    • RBBP5_HUMAN antibody
    • RBQ 3 antibody
    • RBQ3 antibody
    • Retinoblastoma binding protein 5 antibody
    • Retinoblastoma binding protein RBQ3 antibody
    • Retinoblastoma binding protein5 antibody
    • Retinoblastoma-binding protein 5 antibody
    • Retinoblastoma-binding protein RBQ-3 antibody
    • SWD1 antibody
    • SWD1, Set1c WD40 repeat protein, homolog antibody
    see all

Anti-RbBP5 antibody images

  • Anti-RbBP5 antibody (ab87190) at 1 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab87190 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87190, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml

References for Anti-RbBP5 antibody (ab87190)

ab87190 has not yet been referenced specifically in any publications.

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