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Rabbit monoclonal [EPR9905] to RBBP9
Flow Cyt more details Unsuitable for:
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human RBBP9 (Uniprot: O75884)
HepG2, MCF7, and HeLa cell lysates; kidney tissue; MCF7 and HeLa cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20ºC.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.
Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues.
Belongs to the RBBP9 family.
Information by UniProt
B5T overexpressed gene protein antibody
B5T-overexpressed gene protein antibody
Western blot - Anti-RBBP9 antibody [EPR9905] (ab157202)
All lanes : Anti-RBBP9 antibody [EPR9905] (ab157202) at 1/1000 dilution Lane 1 : HepG2 cell lysate Lane 2 : MCF7 cell lysate Lane 3 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP9 antibody [EPR9905] (ab157202)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling RBBP9 using ab157202 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-RBBP9 antibody [EPR9905] (ab157202)
Immunofluorescence analysis of MCF7 cells, labeling RBBP9 using ab157202 at a 1/250 dilution.
Flow Cytometry - Anti-RBBP9 antibody [EPR9905] (ab157202)
Flow cytometry analysis of permeabilized HeLa cells labeling RBBP9 (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"