Recombinant
RabMAb

Anti-RBBP9 antibody [EPR9905] (ab157202)

Overview

  • Product name
    Anti-RBBP9 antibody [EPR9905]
    See all RBBP9 primary antibodies
  • Description
    Rabbit monoclonal [EPR9905] to RBBP9
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human RBBP9 (Uniprot: O75884)

  • Positive control
    • HepG2, MCF7, and HeLa cell lysates; kidney tissue; MCF7 and HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9905
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab157202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.
    • Tissue specificity
      Widely expressed. Expressed at higher levels in tumor tissues than in normal tissues.
    • Sequence similarities
      Belongs to the RBBP9 family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B5T overexpressed gene protein antibody
      • B5T-overexpressed gene protein antibody
      • BOG antibody
      • Bog protein antibody
      • MGC9236 antibody
      • OTTHUMP00000030360 antibody
      • Protein BOG antibody
      • Putative hydrolase RBBP9 antibody
      • RBBP 10 antibody
      • RBBP 9 antibody
      • RBBP-10 antibody
      • RBBP-9 antibody
      • RBBP10 antibody
      • RBBP9 antibody
      • RBBP9_HUMAN antibody
      • Retinoblastoma binding protein 10 antibody
      • Retinoblastoma binding protein 9 antibody
      • Retinoblastoma-binding protein 10 antibody
      • Retinoblastoma-binding protein 9 antibody
      • Retinoma binding protein 9 antibody
      see all

    Images

    • All lanes : Anti-RBBP9 antibody [EPR9905] (ab157202) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 21 kDa
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling RBBP9 using ab157202 at a 1/100 dilution.
    • Immunofluorescence analysis of MCF7 cells, labeling RBBP9 using ab157202 at a 1/250 dilution.
    • Flow cytometry analysis of permeabilized HeLa cells labeling RBBP9 (red), using ab157202 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

    References

    ab157202 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab157202.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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