Overview

  • Product nameAnti-RBCK1 antibody
    See all RBCK1 primary antibodies
  • Description
    Rabbit polyclonal to RBCK1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RBCK1.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; HEK293; HepG2. This antibody gave a positive signal in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab108479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionActs as an E3 ubiquitin-protein ligase, or as part of an E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteoasome. Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates. LUBAC conjugates linear polyubiquitin to IKBKG at 'Lys-285' and 'Lys-309' and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Binds polyubiquitin of different linkage types.
  • Sequence similaritiesContains 1 B box-type zinc finger.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications
    Auto-ubiquitinated. Auto-ubiquitination leads to degradation by the proteasome.
    Phosphorylated. In vitro, phosphorylation inhibits auto-ubiquitination activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf18 antibody
    • Chromosome 20 open reading frame 18 antibody
    • HBV associated factor 4 antibody
    • HBV-associated factor 4 antibody
    • Heme-oxidized IRP2 ubiquitin ligase 1 antibody
    • Hepatitis B virus X associated protein 4 antibody
    • Hepatitis B virus X-associated protein 4 antibody
    • HOIL 1L antibody
    • HOIL-1 antibody
    • HOIL-1L antibody
    • HOIL1 antibody
    • HOIL1L antibody
    • RanBP type and C3HC4 type zinc finger containing 1 antibody
    • RanBP-type and C3HC4-type zinc finger-containing protein 1 antibody
    • RBCC protein interacting with PKC1 antibody
    • Rbck1 antibody
    • RBCK2 antibody
    • RING finger protein 54 antibody
    • RNF54 antibody
    • UB7I3_HUMAN antibody
    • UBCE7IP3 antibody
    • Ubiquitin conjugating enzyme 7 interacting protein 3 antibody
    • Ubiquitin-conjugating enzyme 7-interacting protein 3 antibody
    • XAP3 antibody
    • XAP4 antibody
    • ZRANB4 antibody
    see all

Anti-RBCK1 antibody images

  • All lanes : Anti-RBCK1 antibody (ab108479) at 1 µg/ml (Milk blocking - 5%)

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 102 kDa,24 kDa,32 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab108479 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108479 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RBCK1 antibody (ab108479)

ab108479 has not yet been referenced specifically in any publications.

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