Overview

  • Product name
    Anti-RBM12 antibody
  • Description
    Rabbit polyclonal to RBM12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 625 and 675 of human RBM12 (NP_690051.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 97 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM12 antibody (ab72319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM12 antibody (ab72319)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RBM12 with ab72319 at 1/200 (1µg/ml). Detection: DAB.
  • Western blot - RBM12 antibody (ab72319)
    Western blot - RBM12 antibody (ab72319)
    All lanes : Anti-RBM12 antibody (ab72319) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Predicted band size: 97 kDa
    Observed band size: 117 kDa (why is the actual band size different from the predicted?)

  • Immunoprecipitation - RBM12 antibody (ab72319)
    Immunoprecipitation - RBM12 antibody (ab72319)
    Detection of Human RBM12 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72319 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.

References

ab72319 has not yet been referenced specifically in any publications.

Publishing research using ab72319? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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