Anti-SWAN antibody (ab72319)
Key features and details
- Rabbit polyclonal to SWAN
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SWAN antibody -
Description
Rabbit polyclonal to SWAN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SWAN aa 600-700. NP_690051.1
Database link: Q9NTZ6 -
Positive control
- Whole cell lysate from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 97 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 97 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10137 Human
- Entrez Gene: 75710 Mouse
- Entrez Gene: 652928 Rat
- Omim: 607179 Human
- SwissProt: Q9NTZ6 Human
- SwissProt: Q8R4X3 Mouse
- Unigene: 246413 Human
- Unigene: 27660 Mouse
see all -
Alternative names
- HRIHFB2091 antibody
- KIAA0765 antibody
- RBM 12 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SWAN with ab72319 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-SWAN antibody (ab72319) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 97 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted? -
Detection of Human SWAN by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72319 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72319 has not yet been referenced specifically in any publications.