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Immunogen was a synthetic peptide, which represented a portion of human Coactivator Activator encoded within exon 2 (LocusLink ID 10432).
Our Abpromise guarantee covers the use of ab12325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use.|
Detection of Human CoAA by Western Blot.
Samples: Nuclear extract (NE) from HeLa cells.
Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.