Overview

  • Product name
  • Description
    Rabbit polyclonal to RBM14
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human Coactivator Activator encoded within exon 2 (LocusLink ID 10432).

  • Positive control
    • Nuclear extract from HeLa cells.
  • General notes
    Centrifuge tube to remove product from lid.

Properties

Applications

Our Abpromise guarantee covers the use of ab12325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use.

Target

  • Relevance
    RBM14 is a RNA-binding protein. These proteins contribute to gene expression by regulating the form, abundance and stability of both coding and non-coding RNAs. In vertebrates, RNA binding proteins can also influence many processes involved in RNA processing, for example, activity-dependent transcript localization and localized protein synthesis amongst others.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CoAA antibody
    • Coactivator Activator antibody
    • RBM 14 antibody
    • RBM14 antibody
    • RNA binding motif protein 14 antibody
    • RNA binding protein 14 antibody
    • RRM containing coactivator activator/modulator antibody
    • SIP antibody
    • Synaptotagmin interacting protein antibody
    • SYT interacting protein 1 antibody
    • SYT interacting protein antibody
    • SYT interacting protein SIP antibody
    • SYTIP 1 antibody
    • SYTIP1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 80 kDa

    Detection of Human CoAA by Western Blot.

    Samples: Nuclear extract (NE) from HeLa cells.

    Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Detection of Human CoAA by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab12325 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12325, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your enquiry. The epitope recognized by ab12325 maps to a region between residues 150 and 200 of human Coactivator Activator (RNA Binding Motif Protein 14) using the numbering given in entry NP_006319.1 (GeneID 10432). Unfortunately, I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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