Overview

  • Product nameAnti-RBM14 antibody
    See all RBM14 primary antibodies
  • Description
    Rabbit polyclonal to RBM14
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human Coactivator Activator encoded within exon 2 (LocusLink ID 10432).

  • Positive control
    • Nuclear extract from HeLa cells.
  • General notesCentrifuge tube to remove product from lid.

Properties

Applications

Our Abpromise guarantee covers the use of ab12325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use.

Target

  • RelevanceRBM14 is a RNA-binding protein. These proteins contribute to gene expression by regulating the form, abundance and stability of both coding and non-coding RNAs. In vertebrates, RNA binding proteins can also influence many processes involved in RNA processing, for example, activity-dependent transcript localization and localized protein synthesis amongst others.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • CoAA antibody
    • Coactivator Activator antibody
    • RBM 14 antibody
    • RBM14 antibody
    • RNA binding motif protein 14 antibody
    • RNA binding protein 14 antibody
    • RRM containing coactivator activator/modulator antibody
    • SIP antibody
    • Synaptotagmin interacting protein antibody
    • SYT interacting protein 1 antibody
    • SYT interacting protein antibody
    • SYT interacting protein SIP antibody
    • SYTIP 1 antibody
    • SYTIP1 antibody
    see all

Anti-RBM14 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 80 kDa

    Detection of Human CoAA by Western Blot.

    Samples: Nuclear extract (NE) from HeLa cells.

    Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Detection of Human CoAA by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
  • ICC/IF image of ab12325 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12325, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-RBM14 antibody (ab12325)

This product has been referenced in:
  • Shiratsuchi G  et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . Read more (PubMed: 25385835) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. The epitope recognized by ab12325 maps to a region between residues 150 and 200 of human Coactivator Activator (RNA Binding Motif Protein 14) using the numbering given in entry NP_006319.1 (GeneID 10432). Unfortunately, I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"