Overview

  • Product nameAnti-Rbm15 antibody
    See all Rbm15 primary antibodies
  • Description
    Rabbit polyclonal to Rbm15
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 550 and 600 of human RBM15.

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24100041
WB 1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay be implicated in HOX gene regulation.
  • Involvement in diseaseA chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
  • Sequence similaritiesBelongs to the RRM Spen family.
    Contains 3 RRM (RNA recognition motif) domains.
    Contains 1 SPOC domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • One twenty two protein antibody
    • One-twenty two protein 1 antibody
    • OTT antibody
    • OTT1 antibody
    • Putative RNA-binding protein 15 antibody
    • RBM15 antibody
    • RBM15_HUMAN antibody
    • RNA binding motif protein 15 antibody
    • RNA-binding motif protein 15 antibody
    see all

Anti-Rbm15 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of HUVEC cells labelling RBM15 (red) with ab70549 at 1:100. Cells were fixed with 4% paraformaldehyde in PBS for 5 minutes, permeabilized with 0.5% Triton X-100 in PBS for 5 minutes on ice, blocked with PBS containing 10% BlockAce and incubated with the primary antibodies. An Alexa Fluor 594-conjugated anti-rabbit IgG was used as the secondary antibody. SC35 - green. RMB15 and SC35 are partially co-localized in nuclear speckles and are also present in the the nucleoplasm.

  • All lanes : Anti-Rbm15 antibody (ab70549) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 107 kDa
    Observed band size : 107 kDa


    Exposure time : 3 minutes
  • Detection of Human RBM15 by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining RBM15 in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References for Anti-Rbm15 antibody (ab70549)

This product has been referenced in:
  • Horiuchi K  et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24100041) »

See 1 Publication for this product

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