The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Predicted molecular weight: 107 or 61 kDa. (NCBI AAI03661.1: 61 kDa; UniProt isofom 1: 107 kDa, isoform 2: 105 kDa and isoform 3: 106 kDa)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay be implicated in HOX gene regulation.
Involvement in diseaseA chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Sequence similaritiesBelongs to the RRM Spen family. Contains 3 RRM (RNA recognition motif) domains. Contains 1 SPOC domain.