Overview

  • Product nameAnti-RBM17 antibody
    See all RBM17 primary antibodies
  • Description
    Rabbit polyclonal to RBM17
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 227 and 370 of Human RBM17 (AAH07871).

  • Positive control
    • 293T and Raji cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab101441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.

Target

  • FunctionSplice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
  • Sequence similaritiesContains 1 G-patch domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 45 kDa-splicing factor antibody
    • RBM 17 antibody
    • Rbm17 antibody
    • RNA binding motif protein 17 antibody
    • RNA-binding motif protein 17 antibody
    • SPF 45 antibody
    • SPF45 antibody
    • SPF45_HUMAN antibody
    • Splicing factor (45 kD) antibody
    • Splicing factor (45kD) antibody
    • Splicing factor 45 antibody
    • Splicing factor 45 kDa antibody
    • Splicing factor 45kDa antibody
    see all

Anti-RBM17 antibody images

  • Anti-RBM17 antibody (ab101441) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 45 kDa

References for Anti-RBM17 antibody (ab101441)

ab101441 has not yet been referenced specifically in any publications.

Product Wall

The antibody has not been tested on mouse samples before, so there may be an issue with low cross-reactivity with mouse RBM17, though reactivity is predicted based on homology with the immunogen. In any case, the high background is the main issue.
Read More


In my opinion, the 54 kDa band is phosphorylated RBM17 and the lower 45 kDa band is the dephosphorylated version. The 70 kDa band appears to be a non-specific band.

I came to these conclusions looking at the western blots for two other an...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"