Overview

  • Product name
  • Description
    Rabbit polyclonal to RBM17
  • Tested applications
    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 25-75 of Human RBM17 (NP_116294.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab114968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
    • Sequence similarities
      Contains 1 G-patch domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 45 kDa-splicing factor antibody
      • RBM 17 antibody
      • Rbm17 antibody
      • RNA binding motif protein 17 antibody
      • RNA-binding motif protein 17 antibody
      • SPF 45 antibody
      • SPF45 antibody
      • SPF45_HUMAN antibody
      • Splicing factor (45 kD) antibody
      • Splicing factor (45kD) antibody
      • Splicing factor 45 antibody
      • Splicing factor 45 kDa antibody
      • Splicing factor 45kDa antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RBM17 with ab114968 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of RBM17 by Western Blot of Immunprecipitate.
      anti-RBM17 at 0.4µg/ml staining RBM17 in HeLa whole cell lysate immunoprecipitated using ab114968 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab114968 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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