The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceRBM27 (RNA-binding protein 27) contains one C3H1-type zinc finger and one RRM (RNA recognition motif) domain. The RRM domain mediates integration into cytospeckles.
Cellular localizationCytoplasm. Nucleus speckle. Note: Incorporated into the nuclear speckles and to speckles proximal to the nuclear periphery. Also localizes to punctate structures in the cytoplasm termed cytospeckles.