Overview

  • Product nameAnti-RBM3 antibody
    See all RBM3 primary antibodies
  • Description
    Mouse monoclonal to RBM3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Chimpanzee
  • Immunogen

    Recombinant fragment corresponding to amino acids 1-81 (MSSEEGKLFV GGLNFNTDEQ ALEDHFSSFG PISEVVVVKD RETQRSRGFG FITFTNPEHA SVAMRAMNGE SLDGRQIRVD) of Human RBM3 (NP_006734) with a proprietary tag

  • Positive control
    • Human endometrium tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab118376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 17 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein in western blotting.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.

Target

  • FunctionCold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphoryaltion of translation initiation factors and active polysome formation.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Arg-105 is dimethylated, probably to asymmetric dimethylarginine.
    Phosphorylated.
  • Cellular localizationNucleus. Cytoplasm. Cell projection > dendrite. Localizes in mRNA granules in dentrites.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2600016C11Rik antibody
    • IS1 RNPL antibody
    • MGC105811 antibody
    • MGC118410 antibody
    • OTTHUMP00000025800 antibody
    • OTTHUMP00000025802 antibody
    • OTTMUSP00000019634 antibody
    • OTTMUSP00000019635 antibody
    • OTTMUSP00000019636 antibody
    • Putative RNA binding protein 3 antibody
    • Putative RNA-binding protein 3 antibody
    • Rbm3 antibody
    • RBM3_HUMAN antibody
    • RNA binding motif (RNP1, RRM) protein 3 antibody
    • RNA binding motif protein 3 antibody
    • RNA-binding motif protein 3 antibody
    • RNA-binding protein 3 antibody
    • RNPL antibody
    • RP23-27I6.7 antibody
    see all

Anti-RBM3 antibody images

  • Anti-RBM3 antibody (ab118376) at 5 µg/ml + Recombinant protein fragment at 0.2 µg

    Predicted band size : 17 kDa
  • Formalin-fixed, paraffin-embedded Human endometrium tissue labelled with ab118376 at 3 µg/ml

References for Anti-RBM3 antibody (ab118376)

ab118376 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam earlier today.

I am sorry about the issues you have been having with ab118376 in western blotting. As we discussed I am sending you a vial of ab91291, as a free of charge replacement. Your new order number is *-*...

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