Anti-RBM3 antibody (ab92292)

Overview

  • Product name
  • Description
    Goat polyclonal to RBM3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-DRYSGGNYRDNYDN

    , corresponding to C terminal amino acids 144-157 of Human RBM3 (NP_006734.1).

  • Positive control
    • HepG2 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab92292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 17 kDa.
    An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphoryaltion of translation initiation factors and active polysome formation.
    • Sequence similarities
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Arg-105 is dimethylated, probably to asymmetric dimethylarginine.
      Phosphorylated.
    • Cellular localization
      Nucleus. Cytoplasm. Cell projection > dendrite. Localizes in mRNA granules in dentrites.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2600016C11Rik antibody
      • IS1 RNPL antibody
      • MGC105811 antibody
      • MGC118410 antibody
      • OTTHUMP00000025800 antibody
      • OTTHUMP00000025802 antibody
      • OTTMUSP00000019634 antibody
      • OTTMUSP00000019635 antibody
      • OTTMUSP00000019636 antibody
      • Putative RNA binding protein 3 antibody
      • Putative RNA-binding protein 3 antibody
      • Rbm3 antibody
      • RBM3_HUMAN antibody
      • RNA binding motif (RNP1, RRM) protein 3 antibody
      • RNA binding motif protein 3 antibody
      • RNA-binding motif protein 3 antibody
      • RNA-binding protein 3 antibody
      • RNPL antibody
      • RP23-27I6.7 antibody
      see all

    Images

    • Anti-RBM3 antibody (ab92292) at 0 µg/ml + HepG2 lysate in RIPA buffer at 35 µg

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
      Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    References

    ab92292 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your contact regarding ab92292. I have discussed this enquiry with the Lab and regret to inform you that ab92292 has been tested for Western blot application only. I regret to inform you that unfortunately, we don't have any more test...

    Read More

    Thank you very much for getting back to us. We have now published the Abreviews.

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (C2C12)
    Total protein in input
    30 µg
    Specification
    C2C12
    Treatment
    Cold shock (31.5 C) for 24hrs
    Immuno-precipitation step
    Other - Protein G coated Dynabeads
    Username

    Abcam user community

    Verified customer

    Submitted Oct 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up