Overview

  • Product name
  • Description
    Rabbit polyclonal to RBM33
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    antigen sequence: QHPPQHQHHH HHHHLSVPPP PLMPMSQPQF RPHVQTAQPQ ASSSRMQCPQ RQGLRHNTTS QNVSKRPMQQ MQPTAPRNSN LREL, corresponding to amino acids 628-711 of Human RBM33

  • Positive control
    • Human rectum tissue; RT-4 cells lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/100 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121695 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-RBM33 antibody (ab121695) at 1/100 dilution

    Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
    Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-RBM33 antibody (ab121695) at 1/250 dilution

    Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
    Lane 2 : RT-4 cells lysate
    Lane 3 : U-251 MG cells lysate
    Lane 4 : Human plasma lysate
    Lane 5 : Liver lysate
    Lane 6 : Tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human rectum tissue, using 1/40 ab121695.

References

ab121695 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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