Anti-RBM33 antibody (ab121695)
Key features and details
- Rabbit polyclonal to RBM33
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBM33 antibody -
Description
Rabbit polyclonal to RBM33 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
antigen sequence: QHPPQHQHHH HHHHLSVPPP PLMPMSQPQF RPHVQTAQPQ ASSSRMQCPQ RQGLRHNTTS QNVSKRPMQQ MQPTAPRNSN LREL, corresponding to amino acids 628-711 of Human RBM33
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Positive control
- Human rectum tissue; RT-4 cells lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
Target
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Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. - Information by UniProt
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Database links
- Entrez Gene: 155435 Human
- Entrez Gene: 381626 Mouse
- SwissProt: Q96EV2 Human
- SwissProt: Q9CXK9 Mouse
- Unigene: 591815 Human
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Alternative names
- hypothetical protein MGC20460 antibody
- proline rich 8 antibody
- Proline-rich protein 8 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121695 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-RBM33 antibody (ab121695) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells) -
All lanes : Anti-RBM33 antibody (ab121695) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cells lysate
Lane 3 : U-251 MG cells lysate
Lane 4 : Human plasma lysate
Lane 5 : Liver lysate
Lane 6 : Tonsil lysate -
Immunohistochemical staining of paraffin embedded Human rectum tissue, using 1/40 ab121695.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121695 has not yet been referenced specifically in any publications.