Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RBM34 with ab86787 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - RBM34 antibody (ab86787)
All lanes : Anti-RBM34 antibody (ab86787) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 49 kDa Observed band size: 49 kDa
Exposure time: 10 seconds
Immunoprecipitation - RBM34 antibody (ab86787)
Detection of RBM34 by Western Blot of Immunprecipitate.
ab86787 at 0.4 µg/ml staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86787 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.