Overview

  • Product nameAnti-RBM34 antibody
    See all RBM34 primary antibodies
  • Description
    Rabbit polyclonal to RBM34
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 370 and 430 of human RBM34 (CAI21936.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similaritiesBelongs to the RRM RBM34 family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0117 antibody
    • Rbm34 antibody
    • RBM34_HUMAN antibody
    • RNA binding motif protein 34 antibody
    • RNA-binding motif protein 34 antibody
    • RNA-binding protein 34 antibody
    see all

Anti-RBM34 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RBM34 with ab86787 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-RBM34 antibody (ab86787) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa
    Observed band size : 49 kDa


    Exposure time : 10 seconds
  • Detection of RBM34 by Western Blot of Immunprecipitate.
    ab86787 at 0.4 µg/ml staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86787 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-RBM34 antibody (ab86787)

ab86787 has not yet been referenced specifically in any publications.

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