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Rabbit polyclonal to RBM34
Predicted to work with:
Synthetic peptide, corresponding to a region between residues 75 and 125 of human RBM34 (CAI21936.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab86788 was affinity purified using an epitope specific to RBM34 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 10 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the RRM RBM34 family.
Contains 2 RRM (RNA recognition motif) domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
Western blot - RBM34 antibody (ab86788)
All lanes : Anti-RBM34 antibody (ab86788) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Developed using the ECL technique Predicted band size : 49 kDa Observed band size : 49 kDa Exposure time : 10 seconds
Immunoprecipitation - RBM34 antibody (ab86788)
Detection of RBM34 by Western Blot of Immunprecipitate.
ab86788, at 0.4 µg/ml, staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86788 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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