Overview

  • Product name
  • Description
    Rabbit polyclonal to RBM34
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 75 and 125 of human RBM34 (CAI21936.1).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 10 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Belongs to the RRM RBM34 family.
      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA0117 antibody
      • Rbm34 antibody
      • RBM34_HUMAN antibody
      • RNA binding motif protein 34 antibody
      • RNA-binding motif protein 34 antibody
      • RNA-binding protein 34 antibody
      see all

    Images

    • All lanes : Anti-RBM34 antibody (ab86788) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 49 kDa


      Exposure time : 10 seconds
    • Detection of RBM34 by Western Blot of Immunprecipitate.
      ab86788, at 0.4 µg/ml, staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86788 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    References

    ab86788 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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