Overview

  • Product nameAnti-RBM35A antibody [27H12]
    See all RBM35A primary antibodies
  • Description
    Mouse monoclonal [27H12] to RBM35A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Cow, Dog, Human, Pig, Chimpanzee, Opossum
  • Immunogen

    Full length recombinant Mouse RBM35A with a proprietary tag (NP_918944.2).

  • Positive control
    • RBM35A transfected 293T cell extract
  • General notesStore undiluted; dilute only prior to immediate use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab106585 is an IgG fraction antibody purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered.
  • ClonalityMonoclonal
  • Clone number27H12
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 76 kDa.

Target

Anti-RBM35A antibody [27H12] images

  • All lanes : Anti-RBM35A antibody [27H12] (ab106585) at 1/1000 dilution

    Lane 1 : GFP transfected 293T cell extract
    Lane 2 : RBM35A transfected 293T cell extract
    Lane 3 : RBM35B transfected 293T cell extract

    Lysates/proteins at 5 µg per lane.

    Secondary
    anti-mouse ECL at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 76 kDa

References for Anti-RBM35A antibody [27H12] (ab106585)

This product has been referenced in:
  • Warzecha CC  et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 29:3286-300 (2010). Read more (PubMed: 20711167) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293, HCT116)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 20 µg
Specification HEK293, HCT116
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Simon Boudreault

Verified customer

Submitted Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"