Anti-RBM42 antibody - C-terminal (ab171136)

Overview

  • Product name
    Anti-RBM42 antibody - C-terminal
  • Description
    Rabbit polyclonal to RBM42 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RBM42 aa 393-422 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9BTD8

  • Positive control
    • Human skin tissue; CEM cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171136 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.

Target

  • Relevance
    RNA binding motif protein 42 (RBM42) C-terminal domain interacts with HNRNPK C-terminla domain and RNA. RBM42 and HNRNPK are involved in the maintenance of cellular ATP levels during stress, possibly by protecting critical mRNAs. Upon stress response, RBM42 localizes with HNRNPK in cytoplasmic aggregates of stalled translational pre-initiation complexes called stress granules. There are 4 isoforms produced by alternative splicing.
  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • MGC10433 antibody
    • RNA binding motif protein 42 antibody
    • RNA binding protein 42 antibody

Images

  • Anti-RBM42 antibody - C-terminal (ab171136) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size: 50 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling RBM42 with ab171136 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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