The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
Use at an assay dependent concentration.
FunctionComponent of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
Tissue specificityIsoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines.
Left: human brain tissue stained with ab69770 at 1/50 dilution; Right: the antibody was preincubated with the immunizing peptide.
Western blot - RBM5 antibody (ab69770)
All lanes : Anti-RBM5 antibody (ab69770) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells Lane 2 : Extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size : 92 kDa Observed band size : 92 kDa Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
References for Anti-RBM5 antibody (ab69770)
This product has been referenced in:
Martínez FP & Tang Q Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay. Viruses6:89-105 (2014).
Read more (PubMed: 24385082) »