Overview

  • Product name
  • Description
    Rabbit polyclonal to RBM5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide coresponding to a region within N-terminal residues 1-50 (GSDKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDR Y) of human RBM5 (NP_005769).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
  • Tissue specificity
    Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines.
  • Sequence similarities
    Belongs to the RBM5/RBM10 family.
    Contains 1 C2H2-type zinc finger.
    Contains 1 G-patch domain.
    Contains 1 RanBP2-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ39876 antibody
    • G15 antibody
    • H37 antibody
    • LUCA 15 antibody
    • LUCA15 antibody
    • Protein G15 antibody
    • Putative tumor suppressor LUCA15 antibody
    • RBM 5 antibody
    • Rbm5 antibody
    • RBM5_HUMAN antibody
    • Renal carcinoma antigen NY REN 9 antibody
    • Renal carcinoma antigen NY-REN-9 antibody
    • RMB 5 antibody
    • RMB5 antibody
    • RNA binding motif protein 5 antibody
    • RNA binding protein 5 antibody
    • RNA-binding motif protein 5 antibody
    • RNA-binding protein 5 antibody
    • Tumor suppressor LUCA15 antibody
    see all

Images

  • Anti-RBM5 antibody (ab85504) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 92 kDa
    Observed band size : 102 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 142 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Lv XJ  et al. RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis. Oncol Rep N/A:N/A (2016). Read more (PubMed: 26782095) »
  • Loiselle JJ  et al. Post-transcriptional regulation of Rbm5 expression in undifferentiated H9c2 myoblasts. In Vitro Cell Dev Biol Anim 52:327-36 (2016). Read more (PubMed: 26659391) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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