Overview

  • Product nameAnti-RBP1 antibody [2H3]
    See all RBP1 primary antibodies
  • Description
    Mouse monoclonal [2H3] to RBP1
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human RBP1 produced in HEK293T cells (NP_002890).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY RBP1; COS7 cells transiently transfected with pCMV6-ENTRY RBP1; HeLa cells; Jurkat cells; HEK293T cells transfected with an RBP1 overexpress plasmid; Human liver, ovary, ovary adenocarcinoma and endometrium tissues
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab119056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 16 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionIntracellular transport of retinol.
  • Tissue specificityDetected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular retinol binding protein antibody
    • Cellular retinol-binding protein antibody
    • Cellular retinol-binding protein I antibody
    • CRABP I antibody
    • CRBP antibody
    • CRBP-I antibody
    • CRBP1 antibody
    • CRBPI antibody
    • RBP 1 antibody
    • RBP1 antibody
    • RBPC antibody
    • RET1_HUMAN antibody
    • Retinol binding protein 1 cellular antibody
    • Retinol-binding protein 1 antibody
    see all

Anti-RBP1 antibody [2H3] images

  • All lanes : Anti-RBP1 antibody [2H3] (ab119056) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY RBP1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 16 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC214515)
  • HEK293T cells transfected with either a RBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119056 at 1/100 dilution and then analyzed by flow cytometry.
  • Flow cytometric analysis of RBP1 in Hela cells using ab119056 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
  • Flow cytometric analysis of RBP1 in Jurkat cells using ab119056 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119056, at 1/100 dilution, staining RBP1 in COS7 cells transiently transfected with pCMV6-ENTRY RBP1 by Immunofluorescence.
  • ab119056, at 1/100 dilution, staining RBP1 in Hela cells by Immunofluorescence.
  • ab119056, at 1/150 dilution, staining RBP1 in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab119056, at 1/150 dilution, staining RBP1 in paraffin-embedded Human ovary tissue by Immunohistochemistry.
  • ab119056, at 1/150 dilution, staining RBP1 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab119056, at 1/150 dilution, staining RBP1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.

References for Anti-RBP1 antibody [2H3] (ab119056)

ab119056 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"