Overview

  • Product name
  • Description
    Rabbit polyclonal to RBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 10-103 of Human RBP1 (UniProt ID: P09455).

  • Positive control
    • NIH-3T3 and C2C12 whole cell lysates; H1229 cells; Human Colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Intracellular transport of retinol.
  • Tissue specificity
    Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
  • Sequence similarities
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain
    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular retinol binding protein antibody
    • Cellular retinol-binding protein antibody
    • Cellular retinol-binding protein I antibody
    • CRABP I antibody
    • CRBP antibody
    • CRBP-I antibody
    • CRBP1 antibody
    • CRBPI antibody
    • RBP 1 antibody
    • RBP1 antibody
    • RBPC antibody
    • RET1_HUMAN antibody
    • Retinol binding protein 1 cellular antibody
    • Retinol-binding protein 1 antibody
    see all

Images

  • Anti-RBP1 antibody (ab154881) at 1/5000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 16 kDa



    15% SDS PAGE
  • All lanes : Anti-RBP1 antibody (ab154881) at 1/5000 dilution

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 16 kDa



    12 % SDS-PAGE
  • Immunofluorescence analysis of methanol fixed H1299 cells, labeling RBP1 using ab154881 at 1/500 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded Human colon tissue, labeling RBP1 using ab154881 at 1/100 dilution.

References

This product has been referenced in:
  • Zheng Q  et al. iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery. J Ophthalmol 2015:918473 (2015). WB . Read more (PubMed: 26124962) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Sample
Mouse Cell lysate - whole cell (cortex)
Specification
cortex
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Aug 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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