Anti-RBP1 antibody (ab31106)
Key features and details
- Goat polyclonal to RBP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBP1 antibody
See all RBP1 primary antibodies -
Description
Goat polyclonal to RBP1 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide:
VEGVVCKQVFKKVQ
, corresponding to C terminal amino acids 121-134 of Human PRBPI -
Positive control
- NIH 3T3 lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Ab31106 underwent ammonium sulphate precipitation prior to antigen affinity chromatography purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Intracellular transport of retinol. -
Tissue specificity
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 537379 Cow
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Omim: 180260 Human
- SwissProt: P02694 Cow
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
- Unigene: 529571 Human
see all -
Alternative names
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
Images
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Lane 1 : Anti-RBP1 antibody (ab31106) at 2 µg/ml
Lane 2 : Anti-RBP1 antibody (ab31106) at 0.5 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : U251 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 15 kDa -
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Anti-RBP1 antibody (ab31106) at 0.1 µg/ml + NIH 3T3 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab31106 has been referenced in 2 publications.
- Ludvigsen M et al. Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer. Int J Mol Sci 21:N/A (2020). PubMed: 32422974
- Zhu H et al. Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development. Proteomics 10:3480-93 (2010). WB ; Human . PubMed: 20815088