Overview

  • Product nameAnti-RBP1 antibody
    See all RBP1 primary antibodies
  • Description
    Goat polyclonal to RBP1
  • SpecificityAb31106 recognises RBP1.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    VEGVVCKQVFKKVQ

    , corresponding to C terminal amino acids 121-134 of Human PRBPI

  • Positive control
    • NIH 3T3 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab31106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionIntracellular transport of retinol.
  • Tissue specificityDetected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.
  • Sequence similaritiesBelongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • DomainForms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular retinol binding protein antibody
    • Cellular retinol-binding protein antibody
    • Cellular retinol-binding protein I antibody
    • CRABP I antibody
    • CRBP antibody
    • CRBP-I antibody
    • CRBP1 antibody
    • CRBPI antibody
    • RBP 1 antibody
    • RBP1 antibody
    • RBPC antibody
    • RET1_HUMAN antibody
    • Retinol binding protein 1 cellular antibody
    • Retinol-binding protein 1 antibody
    see all

Anti-RBP1 antibody images

  • Anti-RBP1 antibody (ab31106) at 0.1 µg/ml + NIH 3T3 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 15 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hour
  • ab31106 at 5 µg/ml staining RBP1 in Human pancreas tissue by Immunohistochemistry (Formalin/Paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining was performed.

References for Anti-RBP1 antibody (ab31106)

This product has been referenced in:
  • Zhu H  et al. Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development. Proteomics 10:3480-93 (2010). WB ; Human . Read more (PubMed: 20815088) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. NIH 3T3 lysates were prepared and western blotting done as below. I hope this information helps. Please do not hesitate to contact us if you need anything further. - Lysis. Cell pellets were washed with ice-cold ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"