Overview

  • Product name
  • Description
    Rabbit polyclonal to RBP4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1-151 of Human RBP4 (UniProt ID: P02753).

  • Positive control
    • HepG2 whole cell lysate; HeLa cells; OECM1 xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH 8.0) can also be used.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-RBP4 antibody (ab154914) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded OECM1 xenograft tissue labeling RBP4 with ab154914 at 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling RBP4 with ab154914 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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