Overview

  • Product name
  • Description
    Rabbit polyclonal to RBP4
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RBP4.

    (Peptide available as ab85812.)

  • Positive control
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 24195779
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

Images

  • Anti-RBP4 antibody (ab73022) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg with 3% Milk

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 38 kDa (possible non-specific binding).

    Exposure time : 3 minutes

References

This product has been referenced in:
  • Liu L  et al. Rescue of retinal morphology and function in a humanized mouse at the mouse retinol-binding protein locus. Lab Invest 97:395-408 (2017). Read more (PubMed: 28134916) »
  • Liu LY  et al. Integrating multiple 'omics' analyses identifies serological protein biomarkers for preeclampsia. BMC Med 11:236 (2013). ELISA ; Human . Read more (PubMed: 24195779) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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