Anti-RBPJK antibody (ab217683)
Key features and details
- Rabbit polyclonal to RBPJK
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-RBPJK antibody
See all RBPJK primary antibodies -
Description
Rabbit polyclonal to RBPJK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human RBPJK aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q06330 -
Positive control
- Human gastric carcinoma tissue; Human colon carcinoma lysate; Mouse embryo lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
|
|
WB |
1/100 - 1/1000. Predicted molecular weight: 56 kDa.
|
Notes |
---|
IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 56 kDa. |
Target
-
Function
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. -
Sequence similarities
Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. - Information by UniProt
-
Database links
- Entrez Gene: 3516 Human
- Entrez Gene: 19664 Mouse
- Entrez Gene: 679028 Rat
- Omim: 147183 Human
- SwissProt: Q06330 Human
- SwissProt: P31266 Mouse
- Unigene: 479396 Human
- Unigene: 209292 Mouse
-
Alternative names
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
Images
-
All lanes : Anti-RBPJK antibody (ab217683) at 1/200 dilution
Lane 1 : Mouse embryo lysate
Lane 2 : Human colon carcinoma lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP
Predicted band size: 56 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue, labeling RBPJK with ab217683 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab217683 has not yet been referenced specifically in any publications.